Part:BBa_K4165178

Biobrick GST - Seed

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), pGS-21a RBS (BBa_K4165016), GST tag (BBa_K4165070), Seed peptide (BBa_K4165012), and T7 terminator (BBa_K731721). The GST tag was attached to the Seed peptide coding sequence to serve in the purification using Glutathione resin column.

Usage and Biology

Amyloid 𝛽 seed (37-42) which has the ability to bind to A𝛽 plaques inside the brain. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered main causes of Alzheimer’s Disease pathogenesis.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 763
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 173
  

Dry lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab.

                   Figure 1. this figure shows the results from the transcription and translation code showing the 
                                variation of mRNA and protein concentrations with time.